Device

Part:BBa_K4881028:Design

Designed by: Nyahna Sanchez   Group: iGEM23_FDR-HBPeru   (2023-10-07)


fucO and aldA with AmilGFP reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 251
    Illegal PstI site found at 2012
    Illegal PstI site found at 2725
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2764
    Illegal NheI site found at 2787
    Illegal PstI site found at 251
    Illegal PstI site found at 2012
    Illegal PstI site found at 2725
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1550
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 251
    Illegal PstI site found at 2012
    Illegal PstI site found at 2725
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 251
    Illegal PstI site found at 2012
    Illegal PstI site found at 2725
    Illegal AgeI site found at 967
    Illegal AgeI site found at 1168
    Illegal AgeI site found at 1822
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

aldA and fucO plasmid on Addgene: pEG03 (Plasmid #154138)

References

Panda, S., Fung, V. Y. K., Zhou, J., Hong, L., & Zhou, K. (2021). Improving ethylene glycol utilization in Escherichia coli fermentation. Biochemical Engineering Journal, 168, 107957. https://doi.org/10.1016/j.bej.2021107957